phospho smad3 Search Results


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Biorbyt p smad3 polyclonal antibodies
A , Expression of transforming growth factor (TGF)-β1 in lung tissues in K. pneumoniae rat model treated with astragaloside IV (AS-IV) or TGF-β1 inhibitor SB-431542 detected by immunohistochemistry (scale bar 50 μm); B , Expression levels of proteins related with TGF-β1/Smad, p-Smad2/Smad2, and <t>p-Smad3/Smad3</t> pathways in lung determined by western blot. Data are reported as means±SD (n=3). *P<0.05, **P<0.01 compared with Control group; # P<0.05, ## P<0.01 compared with K. pneumoniae group (ANOVA).
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Abnova smad1
A , Expression of transforming growth factor (TGF)-β1 in lung tissues in K. pneumoniae rat model treated with astragaloside IV (AS-IV) or TGF-β1 inhibitor SB-431542 detected by immunohistochemistry (scale bar 50 μm); B , Expression levels of proteins related with TGF-β1/Smad, p-Smad2/Smad2, and <t>p-Smad3/Smad3</t> pathways in lung determined by western blot. Data are reported as means±SD (n=3). *P<0.05, **P<0.01 compared with Control group; # P<0.05, ## P<0.01 compared with K. pneumoniae group (ANOVA).
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ImmunoWay Biotechnology Company p-smad2/3
A , Expression of transforming growth factor (TGF)-β1 in lung tissues in K. pneumoniae rat model treated with astragaloside IV (AS-IV) or TGF-β1 inhibitor SB-431542 detected by immunohistochemistry (scale bar 50 μm); B , Expression levels of proteins related with TGF-β1/Smad, p-Smad2/Smad2, and <t>p-Smad3/Smad3</t> pathways in lung determined by western blot. Data are reported as means±SD (n=3). *P<0.05, **P<0.01 compared with Control group; # P<0.05, ## P<0.01 compared with K. pneumoniae group (ANOVA).
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Image Search Results


A , Expression of transforming growth factor (TGF)-β1 in lung tissues in K. pneumoniae rat model treated with astragaloside IV (AS-IV) or TGF-β1 inhibitor SB-431542 detected by immunohistochemistry (scale bar 50 μm); B , Expression levels of proteins related with TGF-β1/Smad, p-Smad2/Smad2, and p-Smad3/Smad3 pathways in lung determined by western blot. Data are reported as means±SD (n=3). *P<0.05, **P<0.01 compared with Control group; # P<0.05, ## P<0.01 compared with K. pneumoniae group (ANOVA).

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Astragaloside IV alleviates lung inflammation in Klebsiella pneumonia rats by suppressing TGF-β1/Smad pathway

doi: 10.1590/1414-431X2023e12203

Figure Lengend Snippet: A , Expression of transforming growth factor (TGF)-β1 in lung tissues in K. pneumoniae rat model treated with astragaloside IV (AS-IV) or TGF-β1 inhibitor SB-431542 detected by immunohistochemistry (scale bar 50 μm); B , Expression levels of proteins related with TGF-β1/Smad, p-Smad2/Smad2, and p-Smad3/Smad3 pathways in lung determined by western blot. Data are reported as means±SD (n=3). *P<0.05, **P<0.01 compared with Control group; # P<0.05, ## P<0.01 compared with K. pneumoniae group (ANOVA).

Article Snippet: The primary antibodies were: TGF-β1 polyclonal antibodies (1:800, SAB4502954, Sigma-Aldrich), p-Smad2 polyclonal antibodies (1:100, orb451161, Biorbyt, China), Smad2 polyclonal antibodies (1:100, orb507656, Biorbyt), p-Smad3 polyclonal antibodies (1:100, orb313112, Biorbyt), Smad3 polyclonal antibodies (1:100, orb94696, Biorbyt), NF-κB p-p65 polyclonal antibodies (1:100, orb501839, Biorbyt), NF-κB p65 polyclonal antibodies (1:100, orb344389, Biorbyt), IκBα polyclonal antibodies (1:1000, 4812, Cell Signaling Technology, China), p-IκBα polyclonal antibodies (1:800, 2859, Cell Signaling Technology), and GAPDH polyclonal antibodies (1:1000,5174, Cell Signaling Technology).

Techniques: Expressing, Immunohistochemistry, Western Blot, Control